Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection
نویسندگان
چکیده
منابع مشابه
Detergent-dependent kinetics of truncated Plasmodium falciparum dihydroorotate dehydrogenase.
The survival of the malaria parasite Plasmodium falciparum is dependent upon the de novo biosynthesis of pyrimidines. P. falciparum dihydroorotate dehydrogenase (PfDHODH) catalyzes the fourth step in this pathway in an FMN-dependent reaction. The full-length enzyme is associated with the inner mitochondrial membrane, where ubiquinone (CoQ) serves as the terminal electron acceptor. The lipophili...
متن کاملA set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum.
Genomic information is rapidly accumulating for the human malaria pathogen, Plasmodium falciparum. Our ability to perform genetic manipulations to understand Plasmodium gene function is limited. Dihydrofolate reductase is the only selectable marker presently available for transfection of P. falciparum. Additional markers are needed for complementation and for expression of mutated forms of esse...
متن کاملA set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum
Genomic information is rapidly accumulating for the human malaria pathogen, Plasmodium falciparum. Our ability to perform genetic manipulations to understand Plasmodium gene function is limited. Dihydrofolate reductase is the only selectable marker presently available for transfection of P. falciparum. Additional markers are needed for complementation and for expression of mutated forms of esse...
متن کاملAtovaquone tolerance in Plasmodium falciparum parasites selected for high-level resistance to a dihydroorotate dehydrogenase inhibitor.
Atovaquone is a component of Malarone, a widely prescribed antimalarial combination, that targets malaria respiration. Here we show that parasites with high-level resistance to an inhibitor of dihydroorotate dehydrogenase demonstrate unexpected atovaquone tolerance. Fortunately, the tolerance is diminished with proguanil, the second partner in Malarone. It is important to understand such "genet...
متن کاملHigh-throughput screening for potent and selective inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase.
Plasmodium falciparum is the causative agent of the most serious and fatal malarial infections, and it has developed resistance to commonly employed chemotherapeutics. The de novo pyrimidine biosynthesis enzymes offer potential as targets for drug design, because, unlike the host, the parasite does not have pyrimidine salvage pathways. Dihydroorotate dehydrogenase (DHODH) is a flavin-dependent ...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2011
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2011.01.004